Author: marshallclune

Today we have prepared a solution on which we will run PCR. This solution contains DNA from a sample we gathered last week. PCR uses a series of temperature shifts to replicate the quantity of the DNA exponentially. The PCR process takes some time so we are letting the machine run in between our class periods. I will be gone next week for Fall Break, so Dr. Ren has set aside my other samples that I will run PCR on the following week.

Today in class we are extracting DNA from a sample of Daphnia Magna. The process involves several additions of various buffers and decanting the solution left in the bottom of the collection tube after centrifuging. Most recently, we added 50 microliters of Buffer AE to the center of the spin column membrane (little white circle inside the tube) and are waiting for a 15 minute incubation period to be over. Just the right amount of time to let the world peek into another Thursday morning in Life Science West.

Last week in class, we gave presentations over various topics pertaining to DNA barcoding. For example, my group’s topic was the development of DNA sequencing techniques. It was interesting to learn from my peers about these different subjects as well as receive critiques from Dr.’s Ren and French on our presentation and poster design. On a lighter note, we also received our very own OSU printed name tags, which adds a feeling of being a little more official here on campus.